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Novel immunization stratagies, antigen design, and screening technologies are essential for the successful generation of monoclonal hybridoma cells lines. Our immunization protocols take advantage of using multiple strains of mice along with altered immunization routes, combinations of ligand carriers and dosing strategies to produce as diverse of collective monoclonal antibody population as possible. This polyclonal utility is essential because diversity of clones to immortalization is a major limiting factor in traditional monoclonal generation. Antigens must be carefully designed, especially when dealing with peptides, to generate a robust reagent that is able to recognize conformational changes of the native protein in assay conditions. Click on our different protocol options below to see which option is best for your needs.
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Our staff has produced more than 16,000 monoclonal and polyclonal antibodies. We have designed a series of novel protocols to optimize antigen presentation conditions and assay formatting to ensure the highest success rate possible. This experience offers you access to our antigen design and conjugation methods.
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