Recommended for highly purified recombinant proteins and peptide-conjugates where antibodies will be used primarily in western blotting or IHC, but are also needed for multiple applications where epitope structure is extremely important (IP, capture, etc.).1) Immunization of 5 SW and 5 Balb/c (10 mice total) over 11 week protocol2) Primary Immunization, 3 boosts, test-bleed, ELISA titration*3) Fusion of best three mice by ELISA and/or customer evaluation of crude antiserum4) Screening by ELISA-based assay (negative screen included)5) Evaluation of positive sups by customer6) Expansion and freeze down of parental cell lines7) Deliver of up to 10 best parental cell lines as frozen stocks
Total production time is approximately 4-6 months. Development is broken down into three phases, which defer costs at each step. *Depending on the initial ELISA results, the mice may need additional boosts and bleeds in order to generate titers that meet our minimum requirement for fusions. This extension service is offered at no additional cost.
For more hybridoma options, please visit Custom Monoclonal Antibodies. Open Biosystems also offers Additional Monoclonal Services such as cell culture, monoclonal production, ascites production and purification. |
