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5 Mouse Monoclonal Antibody

Recommended for highly purified recombinant proteins where antibodies will be used primarily in western blotting, IP, or ELISA.  Not recommended for peptide antigens or small proteins (<9kDa).
  
Description


1)       Immunization of 5 SW mice over 11 week protocol
2)       Primary Immunization, 3 boosts, test-bleed, ELISA titration*
3)       Fusion of best two mice by ELISA and/or customer evaluation of crude antiserum
4)       Screening by ELISA-based assay
5)       Evaluation of positive sups by customer
6)       Expansion and freeze down of parental cell lines
7)       Deliver of up to 5 best parental cell lines as frozen stocks 
 
Total production time is approximately 4-6 months.  Development is broken down into three phases, which defer costs at each step.
 
*Depending on the initial ELISA results, the mice may need additional boosts and bleeds in order to generate titers that meet our minimum requirement for fusions.  This extension service is offered at no additional cost.   

For more hybridoma options, please visit Custom Monoclonal Antibodies.  Open Biosystems also offers Additional Monoclonal Services such as cell culture, monoclonal production, ascites production and purification. 


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