2 Goat, 120 Day Protocol
 
   

Procedure

Protocol Day*

Description

Antigen preparation

 

Peptide synthesis and purification (if required), antigen conjugation

Control serum Collection

Day 0

 

Primary injection**

 

Day 1

Primary Immunization with 0.5 mgs KLH emulsified with Freund's complete adjuvant, ID 20 sites

1st Booster

Day 14

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

2nd Booster

Day 28

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

Serum Collection

Day 42

Test-bleed (~5mls)

3rd Booster

Day 56

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

Serum Collection

Day 56

Test-bleed (~5mls)

4th Booster

Day 70

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

5th Booster

Day 84

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

Serum Collection

Day 84

Test-bleed (~5mls)

6th Booster

Day 98

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

7th Booster

Day 112

Boost with 0.50mgs of KLH emulsified with Freund's complete adjuvant, ID 20 sites

Serum Collection

Day 112

Test-bleed (~5mls)

ELISA and Shipping

Day 118

One ~1100ml plasmaphoresis bleed taken upon customer request.  Extension protocol is recommended with titer monitoring by ELISA

Instruction Due Date

Day 120

Termination of animal if instructions not received

 

* Days listed are approximate (± 2 days).

** Two goats will be utilized for each MAP-peptide, conjugated-peptide, or protein immunogen.

*** Continuation of goats is offered on a month to month basis.  The standard extension protocol includes seven boosts, five
serum collections and maintenance for one month.  Production bleeds may be obtained atanytime yielding approximately 250 mls
of additional sera per bleeding. 
  
© 2005-2007 Open Biosystems All Rights Reserved