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2 Rabbit, 70 Day Protocol

Procedure	Protocol Day*	Description
Antigen Preparation		Peptide synthesis and purification (if required) and antigen conjugation
Control Serum Collection	Day 0	Bleed 10 mL per rabbit
Primary injection**	Day 1	Primary Immunization with 0.50 mg of antigen in 10 SQ sites
1st Booster	Day 14	Boost with 0.25 mg of antigen in 4 SQ sites
2nd Booster	Day 28	Boost with 0.25 mg of antigen in 4 SQ sites
Serum Collection	Day 35	Bleed ~ 25 mL per rabbit
3rd Booster	Day 42	Boost with 0.25 mg of antigen in 4 SQ sites
Serum Collection	Day 56, 58	Two production bleeds ( ~ 50 mL total per rabbit)
ELISA and Shipping	Day 60	Verify disposition of rabbits, continue*** or terminate
Instruction Due Date	Day 72	Termination of animals if instructions not received

* Days listed are approximate (± 2 days).

** Two New Zealand white rabbits will be utilized for each MAP-peptide, conjugated-peptide immunogen, or protein immunogen and are house

in individual cages.

*** Continuation of rabbits is offered on a month to month basis. The standard extension protocol includes one boost, one serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately 50mls of additional sera per rabbit.

For more protocol options, please visit Polyclonal Animal Protocols.

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