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2 SPF Rabbit, 90 Day Protocol

Procedure	Protocol Day*	Description
Antigen preparation		Peptide synthesis and purification (if required), antigen conjugation
Control serum Collection	Day 0
Primary injection**	Day 1	Primary Immunization with 0.50 mg MAP or 0.25 mg KLH emulsified with Freund's complete adjuvant, SQ 4 sites
1st Booster	Day 14	Boost with 0.50 mg MAP or 0.10 mg KLH emulsified with Freund's incomplete adjuvant.
Serum Collection	Day 28	~25mls per rabbit
2nd Booster	Day 42	Boost with 0.50 mg MAP or 0.10 mg KLH emulsified with Freund's incomplete adjuvant.
Serum Collection	Day 56	~25mls per rabbit
3rd Booster	Day 56	Boost with 0.50 mg MAP or 0.10 mg KLH emulsified with Freund's incomplete adjuvant.
Serum Collection	Day 70	Large-volume production bleeds (~50mls per rabbit)
ELISA and Shipping	Day 77	Verify disposition of rabbits, Continue*** or Term
Instruction Due Date	Day 90	Termination of animals if instructions not received

* Days listed are approximate (± 2 days).

** Two New Zealand white rabbits will be utilized for each MAP-peptide, conjugated-peptide immunogen, or protein immunogen and are house

in individual cages.

*** Continuation of rabbits is offered on a month to month basis. The standard extension protocol includes one boost, one serum collection, and maintenance for one month. Terminal bleeds may be obtained at any time yielding approximately 50mls of additional sera per rabbit.

For more protocol options, please visit Polyclonal Animal Protocols.

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