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5 Mouse, 80 Day Protocol

Procedure	Protocol Day*	Description
Antigen preparation		Peptide synthesis and purification (if required), antigen conjugation
Control serum Collection	Day 0
Primary injection**	Day 1	Primary Immunization of 0.1mgs emulsified with Freund's complete adjuvant, SQ 4 sites
1st Booster	Day 21	Boost with 50µg in IFA, SQ 4 sites
2nd Booster	Day 42	Boost with 50µg in IFA, SQ 4 sites
Test-bleed	Day 50	Test-bleed (~1ml per animal)
ELISA and shipping	Day 60	ELISA titration of pre-immune and test-bleed
3rd Booster	Day 62	Boost with 50µg in IFA, SQ 4 sites
Continue or Exsanguinate	Day 64	Verify disposition, extend or terminate.
Instruction Due Date	Day 80	Termination of animals if instructions not received

* Days listed are approximate (± 2 days).

** Five mice will be utilized for each MAP-peptide, conjugated-peptide immunogen, or protein immunogen and are house

in individual cages.

*** Continuation of mice is offered on a month to month basis. The standard extension protocol includes one boost, one serum collection, and maintenance for one month. Exsanguinatory bleeds may be obtained at any time yielding approximately 0.5-1ml of additional sera per mouse.

For more protocol options, please visit Polyclonal Animal Protocols.

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