Antibodies to discrete epitopes can be isolated and removed from larger polyclonal populations through affinity enrichment and depletion. This is especially important when producing antibodies against protein modification such as phosphorylation and acetylation. Open Biosystems has developed cutting edge capabilities in chromatography that enable the most specific and highest affinity populations of antibodies to be separated from polyclonal pools to create a more robust yet specific protein detection reagent. Our antibody purifications services include the following:
- Peptide Affinity
- Peptide Negative Adsorbtion (primarily used for modification specific antibodies)
- Protein Affinity (3-5 mg of additional protein required)
- Protein A/G (high salt conditions)
- IgY Isolation (PEG precipitation)
- Ammonium Sulfate
- Antibodies are isolated and exposed to depletion columns to remove unwanted binders and enrich for target antibodies
- pH elution gradient is used to capture diverse polyclonal species and minimize antibody pH labiality
- Affinity ligands are bound to matrices through multiple attachment points mimic antigen presentation in vivo increasing capturing efficiency.
