The Boone lab at the University of Toronto has developed a collection of over 4900
Saccharomyces cerevisiae (S288C) Open Reading Frames (ORFs) expressed from its endogenous promoter and tagged with a unique molecular barcode. The MoBY ORF Collection enables simultaneous measurement of all transformants in a single pooled culture using a barcode microarray readout. The collection is available as a complete set as glycerol stocks in 96-well plate format.
Applications:• Genome scale parallel screening
• Discover mode-of-action for drug discovery and mutation screening
Features:• Genome-scale - >4,900 uniquely barcoded ORFs
• Dual barcodes - Increased hit confidence
• Native context - Sequence upstream and downstream of the ORF
• Endogenous promoter - Biologically relevent expression levels
• Easy selection - KanMX resistance cassette on plasmid backbone
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