Mouse embryonic stem (ES) cells are now available through Open Biosystems. We provide six different ES cell lines of various strain backgrounds, developed in the renowned lab of Dr. Rudolph Jaenisch, co-founder of the Whitehead Institute. These include two hybrid ES cell lines C57BL/6 X 129/sv and BALB/c X 129/sv that allow tetraploid injections. All strains of Mouse ES cells from Open Biosystems have been used successfully in producing germline transmitting chimeras.
Mouse ES cells are derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo (i.e. the blastocyst). Pluripotency is a special quality of ES cells that gives them the potential to develop into any cell types, or produce a whole animal. Mouse ES cell lines can be used in the creation of mouse models, the tool of choice for determining the function of your sequence of interest in the context of a whole organism, its role in disease, and the generation and testing of therapeutics. They are amenable to one of the most powerful methods of genetic engineering: Gene targeting. This method allows the researcher to make precise modifications to their sequence of interest and then generate a mature adult mouse carrying the engineered alteration. The utility of this technique reaches far beyond the familiar knockout mouse. Its applications include the creation of mutations that mimic human disease, probing for the active site(s) of proteins, investigation of protein/protein interactions, determining the role of regulatory sequences, and much more. ES cells are also useful in cell culture studies. Their ability to maintain a normal karyotype for innumerable cell divisions as well as their untransformed status makes them especially appealing. Various protocols have been published showing in vitro differentiation of mouse ES cells into many cell types including neuronal, cardiac and epithelial.
Open Biosystems is committed to providing a variety of high quality mouse ES cells at a low cost. Take advantage of our no strings attached policy, and rapid delivery. See our growing stock of mouse ES cells in the table below, and order today.
Technology Access Fee
The purchase of any murine ES cell line by for-profit entities requires payment of a technology access fee. This one time charge provides the purchaser with the rights to further utilize all available Open Biosystems murine ES cell lines without limitation. This one time access fee goes back to the Jaenisch laboratory at the Whitehead Institute. It is used for funding postdoctoral research fellows. Contact the customer service team at info@openbiosystems.com or 1-888-412-2225 for details.
The references below should be used to cite the creation of the technology. Do not contact the source lab for these products. Please direct ALL product question to our technical support team.
Eggan K, et al . (2001). Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. 2001 May 22;98(11):6209-14.
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Buchholz F, et al . (1998). Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature Biotechnology 16 , 657–662.
Hochedlinger K, et al . (2005). Ectopic expression of Oct-4 blocks Progenitor cell differentiation. Cell 121 , 465–477.
Urlinger S, et al . (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc.Natl. Acad. Sci. USA 97 , 7963–7968.
